Introduction: ORA-AID adhesive is recognized as an effective dressing for dental sockets in adults, yet its effectiveness in adolescents has not been explored. This study compared ORA-AID with conventional dressings in the patients younger than 18. Methods: A double-blind clinical trial was conducted involving 29 patients. On one side, a tooth was extracted and dressed using a standard method. After two weeks, the tooth on the other opposite side was extracted, and the ORA-AID adhesive was utilized. Patients reported their pain levels using the Visual Analog Scale (VAS) at 5, 12, 24, and 48 hours following the extraction. Repeated Measures ANOVA was employed for comparisons between the two methods. Results: Pain was significantly lower with ORA-AID, except for the first 5 hours (p=0. 669), and remained and remained so for up to 48 hours after extraction (all p<0. 001). Increased pain levels were observed in older patients (24 and 48 hours) as well as in individuals with greater dental plaque (5 and 12 hours). The highest decrease in pain with ORA-AID in comparison to conventional dressing was observed at 24 hours after extraction. Conclusion: ORA-AID significantly reduces post-extraction pain when compared to conventional dressings, except for the initial 5 hours. Therefore, ORA-AID may reserve as an appropriate alternative for routine dental dressings in adolescents.

Background and purpose: Activation-induced cytidine deaminase (AID) is a B-cell specific enzyme responsible for somatic hypermutation (SHM) and class switch recombination (CSR) of antibody genes within the B-cell follicle of peripheral lymphoid organs. Ectopic overexpression of the enzyme leads to mutations in non-B cells and Escherichia coli (E. coli) genes. However, induction of mutations in E. coli by expression of AID requires highly regulated conditions. Therefore, the aim of this study was to construct a plasmid expressing AID under the control of tightly regulated temperature-sensitive promoter of bacteriophage lambda. . Materials and methods: The AID gene was isolated from PGEMT recombinant plasmid, containing AID and cloned into PTG19-T vector. Then, AID was ligated to an expression plasmid under the control of left promoter of bacteriophage lambda and mutant thermolabile cI857 repressor. Finally, the resistance marker of the engineered plasmid was replaced by tetracycline resistance gene. Results: The whole open reading frame of AID was ligated into a plasmid containing a cI857-sensitive receptor lambda bacteriophage. The sequence and reading frame accuracy of the cloning was confirmed by electrophoresis of PCR products on agarose gel, restriction enzyme digestion, and nucleotide sequencing methods. Conclusion: In current study, the AID was successfully cloned into an expression plasmid containing thermo-sensitive promoter of bacteriophage lambda. The recombinant plasmid could be used in different strains of E. coli to produce mutator strains.